甘肃省白菜死棵病病原菌鉴定及其融合群检测技术

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王朵,谢学文,柴阿丽,石延霞(

),李宝聚(

)

Identification of the Pathogen Causing Cabbage Died in Gansu Province and Detection of Anastomosis Groups

WANG Duo,XIE XueWen,CHAI ALi,SHI YanXia(

),LI BaoJu(

)

图3. 特异性引物F-Common/R-AG-2-1和F-Common/R-AG-1-IB对分离菌株的扩增产物电泳图

Fig. 3. PCR products of R. solani strains collected from cabbage in Gansu Province using specific primer pairs of F-Common/ R-AG-2-1 and F-Common/R-AG-1-IB
M：DNA分子量标准（5 000 bp） DNA marker (5 000 bp);1—8：F-Common/R-AG-2-1特异性扩增 Amplification by primer F-Common/R-AG-2-1 for AG-2-1;9—17：立枯丝核菌融合群AG-3—AG-11菌株 R. solani AG3 to AG11;18：阴性对照 Negative control;19—23：F-Common/R-AG-1-IB特异性扩增 Amplification by primer F-Common/R-AG-1-IB for AG-1-IB