甘肃省白菜死棵病病原菌鉴定及其融合群检测技术

王朵,谢学文,柴阿丽,石延霞,李宝聚

Identification of the Pathogen Causing Cabbage Died in Gansu Province and Detection of Anastomosis Groups

Duo WANG,XueWen XIE,ALi CHAI,YanXia SHI,BaoJu LI

表4 Real-time PCR检测人工接种植株及根际土壤的带菌量

Table 4 The detection results of real-time PCR of infected cabbage samples and rhizosphere soils

接种后天数 Days after inoculation (d) 平均Ct 值 The average cycle threshold value DNA平均拷贝数的对数值 Logarithm of average copy number of DNA (lg copies/μL) 茎基部 Stem 根际土壤 Soil 茎基部 Stem 根际土壤 Soil 1 23.46 31.28 5.85 3.66 5 11.92 18.84 9.11 7.16 10 20.86 23.57 6.59 5.76 15 28.92 26.88 4.31 4.90 20 30.40 32.85 3.90 3.21