甘肃省白菜死棵病病原菌鉴定及其融合群检测技术

王朵,谢学文,柴阿丽,石延霞,李宝聚

Identification of the Pathogen Causing Cabbage Died in Gansu Province and Detection of Anastomosis Groups

Duo WANG,XueWen XIE,ALi CHAI,YanXia SHI,BaoJu LI

表1 本研究中所用融合群鉴定的引物序列及其来源

Table 1 Primers used in this study with sequences and sources

基因 Gene	片段长度 Fragment length (bp)	引物名称 Primer name	方向 Direction	序列 Sequence	引物来源 Primer source
ITS	700	ITS1	Forward	TCCGTAGGTGAACCTGCGG	文献[22] Reference [22]
ITS	700	ITS4	Reverse	TCCTCCGCTTATTGATATGC	文献[22] Reference [22]
TEF-1α	680	RSF	Forward	CGTGAYTTYATCAAGAACATG	文献[23] Reference [23]
TEF-1α	680	RSR	Reverse	GACTTGACTTCAGTGGTCAC	文献[23] Reference [23]
28S		F-Common	Forward	CTCAAACAGGCATGCTC	文献[24] Reference [24]
	300	R-AG-2-1	Reverse	AGGCAATAGGTTATTGGACC
	250	R-AG-1-IB	Reverse	AAGGTCCTTTGGGGTTGGGG