基于全基因组重测序的大豆分子标记开发及籽粒蛋白质含量QTL定位
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王嘉,曾召琼,梁建秋,于晓波,吴海英,张明荣(  )
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Development New Molecular Markers for Quantitative Trait Locus (QTL) Analysis of the Seed Protein Content Based on Whole Genome Re-Sequencing in Soybean
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WANG Jia,ZENG ZhaoQiong,LIANG JianQiu,YU XiaoBo,WU HaiYing,ZHANG MingRong(  )
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图3. CAPS标记和InDel标记开发及多态性分析 a:靶向Gy1含限制性酶切位点BanⅡ的SNP部分序列比对结果。线条代表标注出的限制性内切酶识别位点,箭头代表酶切位点;b:南豆12和十月黄中BBX21包含InDel的部分序列比对结果;c:靶向Gy1的CAPS标记酶切检测结果;d:靶向BBX21的InDel标记的琼脂糖凝胶电泳结果。S:Shiyuehuang,N:南豆12,1—10:部分F2材料
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Fig. 3. Development and polymorphism analysis of CAPS and InDel markers a: Alignment of the partial sequence of containing the SNP which resulted in the creation of the restriction sites (BanⅡ) between Nandou 12 and Shiyuehuang in Gy1. Lines represent the resriction sites, while arrows indicate the position of digesting site; b: Sequence alignment results of BBX21 gene containing InDel in Nandou 12 and Shiyuehuang; c: Detection of CAPS labeled enzyme digestion targeting Gy1 gene; d: The results of InDel markers amplification for targeting BBX21 gene were analyzed by agarose gel electrophoresis. S: Shiyuehuang, N: Nandou 12, Figure exhibited partial verifying lines (code number: 1-10)
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