基于全基因组重测序的大豆分子标记开发及籽粒蛋白质含量QTL定位

王嘉,曾召琼,梁建秋,于晓波,吴海英,张明荣

Development New Molecular Markers for Quantitative Trait Locus (QTL) Analysis of the Seed Protein Content Based on Whole Genome Re-Sequencing in Soybean

Jia WANG,ZhaoQiong ZENG,JianQiu LIANG,XiaoBo YU,HaiYing WU,MingRong ZHANG

表2 南豆12和十月黄之间出现非同义替换最多的前10个基因

Table 2 Top 10 genes that are highly polymorphic between Nandou 12 and Shiyuehuang by non-syn SNPs

基因ID Gene ID 基因名称 Gene name 注释 Annotation 基因区域内的SNP数目 Genic SNP CDS中的SNP SNP in CDS 非同义替换SNP Non-syn SNP Glyma.18G176100 ABI3 ABI3-like转录因子家族成员,促进种子储藏蛋白的积累 A member of ABI3-like transcription factor family, promoting the accumulation of seed storage proteins 72 31 20 Glyma.13G123500 Gy5 大豆球蛋白基因家族成员之一,编码一个11S球蛋白亚基 Encodes an 11S globulin subunit, a member of the soybean globulin gene family 46 33 18 Glyma.11G112800 BBX21 编码B-box锌指转录因子bbx21,与cop1基因相互作用调节避荫 Encodes a B-box zinc finger transcription factor BBX21, genetically interacts with COP1 to regulate shade avoidance. 30 29 16 Glyma.18G175500 MAG2 参与种子贮藏蛋白从内质网到液泡的运输 Involved in transportation of seed storage proteins from the ER to the vacuole 36 27 14 Glyma.13G335200 OBP3 编码一个主要在根中表达的包含转录因子的核定位Dof域 Encodes a nuclear localized Dof domain containing transcription factor expressed primarily in roots. 35 35 14 Glyma.02G057500 SAV1 编码一个油菜素类固醇生物合成途径的限速酶22α羟化酶 Encodes a 22α hydroxylase whose reaction is a rate-limiting step in brassinosteroid biosynthetic pathway 51 29 12 Glyma.08G171800 GA2OX4 编码作用于赤霉素C19的赤霉素2-氧化酶 Encodes a gibberellin 2-oxidase that acts on C19 gibberellins 88 26 11 Glyma.20G048500 VPS26B 细胞内蛋白转运 Intracellular protein transport 32 25 11 Glyma.18G226500 CRA1 编码一个12S种子储存蛋白 Encodes a 12S seed storage protein 72 26 10 Glyma.14G062100 COI1 避荫 Shade avoidance 28 25 10