基于全基因组重测序的大豆分子标记开发及籽粒蛋白质含量QTL定位

王嘉,曾召琼,梁建秋,于晓波,吴海英,张明荣

Development New Molecular Markers for Quantitative Trait Locus (QTL) Analysis of the Seed Protein Content Based on Whole Genome Re-Sequencing in Soybean

Jia WANG,ZhaoQiong ZENG,JianQiu LIANG,XiaoBo YU,HaiYing WU,MingRong ZHANG

表1 qRT-PCR验证基因引物信息

Table 1 Primer information of candidate genes

基因ID Gene ID 基因名称 Gene name 正向引物 Forward primer (5'-3') 反向引物 Reverse primer (5'-3') Glyma.18G176100 ABI3 CTCATCATGCAAATCCTACAGC CTATCTGATGCAACTCGACTCT Glyma.13G123500 Gy5 CAGATATAGAGCACCCAGAGAC GGTGTTGAAGGATTGTGCTAAG Glyma.18G175500 MAG2 CAAATTTGGGGCATGGCTAATA CTTGAAAATTGTTTCGGTTGCG Glyma.18G226500 CRA1 CTGCAAGAAGTGGTCGTTTTTA ATTTCAGTTGTTCCATGTTCCG Glyma.02g248300 PAT2 AAAGGCGTTGAAGCTGGATATA AATCTCCTATCTGGATCTCCGA Glyma.11g112800 BBX21 CACAACAGGTTTCTTCTCACTG TGTTCTTTCTTCAATGGCAGTG Glyma.14g062100 COI1 GTACATACACGACTCCAAGGAC TTTCCCTTCAGGTTTAACGACT Glyma.05g062700 SAV1 GTGGGTCTCCAATGTTTTGTAC GCAAATTGTTTCAATGGGTGTG Glyma.18g043000 KAN TTGGAGTTCACATTAGGGAGAC AAGTTTGTCACATGTTACCGTG Glyma.03g227300 PHYA CAAAGTCTGATAGAGCAGCAAC CTCACTGTAAGCGATGACCTTA ACT11 CGGTGGTTCTATCTTGGCATC GTCTTTCGCTTCAATAACCCTA