产苦马豆素疯草内生真菌Alternaria Section Undifilum oxytropis的诱变筛选

郝宝成,宋向东,高艳,王学红,刘宇,李元曦,梁妍,陈柯源,胡毓瑶,邢小勇,胡永浩,梁剑平

Mutagenesis and Screening of Endophytic Fungus Alternaria Section Undifilum oxytropis Producing Swainsonine from Locoweed

BaoCheng HAO,XiangDong SONG,Yan GAO,XueHong WANG,Yu LIU,YuanXi LI,Yan LIANG,KeYuan CHEN,YuYao HU,XiaoYong XING,YongHao HU,JianPing LIANG

表5 紫外辐照与亚硝基胍复合诱变对菌株的致死率

Table 5 Lethal rate of Alternaria Section Undifilum oxytropis strains by ultraviolet irradiation-NTG mutagenesis (n=6)

紫外辐照时间 Ultraviolet irradiation time (s) 亚硝基胍剂量 Nitroguanidine dose (μL) 诱变时间 Mutagenesis time (min) 诱变后菌株生长数 Number of strains after mutagenesis 对照组 Control group 致死率 Lethal rate (%) 20 2 5 6 5 6 6 4 5 13 58.98±6.28 40 2 5 3 2 2 4 3 3 12 76.39±6.27 80 2 5 1 0 1 0 0 2 12 94.45±6.80 160 2 5 0 0 2 0 0 1 13 96.15±6.43 320 2 5 0 0 0 0 0 0 11 100.00±0.00 640 2 5 0 0 0 0 0 0 13 100.00±0.00