产苦马豆素疯草内生真菌Alternaria Section Undifilum oxytropis的诱变筛选

郝宝成,宋向东,高艳,王学红,刘宇,李元曦,梁妍,陈柯源,胡毓瑶,邢小勇,胡永浩,梁剑平

Mutagenesis and Screening of Endophytic Fungus Alternaria Section Undifilum oxytropis Producing Swainsonine from Locoweed

BaoCheng HAO,XiangDong SONG,Yan GAO,XueHong WANG,Yu LIU,YuanXi LI,Yan LIANG,KeYuan CHEN,YuYao HU,XiaoYong XING,YongHao HU,JianPing LIANG

表3 亚硝基胍诱变对Alternaria Section Undifilum oxytropis菌株致死率

Table 3 Lethal rate of Alternaria Section Undifilum oxytropis by nitrosoguanidine mutagenesis (n=6)

诱变处理时间 Mutagenesis time（min） 亚硝基胍剂量 Nitroguanidine dose (μL) 空白对照 Blank control 2 4 6 8 10 12 5 8.33±5.38 33.33±14.05 58.33±4.59 75.00±3.72 91.67±13.29 100.00±0.00 0 10 33.33±7.38 66.67±13.66 75.0±22.36 91.67±7.52 91.67±3.50 100.00±0.00 0 15 58.33±7.46 75.00±6.85 91.67±6.05 100.0±0.00 100.0±0.00 100.00±0.00 0 20 75.00±3.95 91.67±7.45 100.0±0.00 100.0±0.00 100.00±0.00 100.00±0.00 0 25 83.33±12.11 100.00±0.00 100.0±0.00 100.00±0.00 100.00±0.00 100.00±0.00 0 30 91.67±5.31 100.0±0.00 100.0±0.00 100.00±0.00 100.00±0.00 100.00±0.00 0