慢病毒介导shRNA干扰MAT2A与MAT2B基因抑制猪肌内脂肪细胞分化
赵存真,易本驰,陈培荣,李建柱,赵云焕,朱忠珂(
)
)
Lentivirus Mediated Interference Silencing MAT2A and MAT2B Inhibited Differentiation of Porcine Intramuscular Preadipocytes
ZHAO CunZhen,YI BenChi,CHEN PeiRong,LI JianZhu,ZHAO YunHuan,ZHU ZhongKe()
)
图3. sh-MAT2A/2B重组慢病毒感染猪前体脂肪细胞的效率
A-C: sh-scaramble, sh-MAT2A 和 sh-MAT2B侵染猪肌内脂肪细胞72 h的荧光表达。D-E: Real-time qPCR 检测MAT2A和MAT2B基因的干扰效率
Fig. 3. The efficiency of cultured preadipocytes that were infected with recombinant lentivirus
A-C Porcine preadipocytes were separately infected by sh-scaramble (as a control), sh-MAT2A and sh-MAT2B for 72 h. D-E: Real-time qPCR to analyze the relative expression of MAT2A and MAT2B (Data are presented as means ± SEM, n=3. *P<0.05, **P<0.01)
