图1. 重组慢病毒干扰载体的构建与鉴定
(A)：琼脂糖凝胶电泳检测合成的shRNA退火所形成双链。1-3：3条sh-MAT2A形成的双链;4-6：3条sh-MAT2B形成的双链。(B) ：LentiH1 载体的Xho I和BamH I双酶切检测。(C)：重组质粒的琼脂糖凝胶电泳酶切鉴定。1,3,5,7,9,11经酶切的质粒鉴定,2, 4, 6, 8, 10, 12未经过酶切的质粒鉴定

Fig. 1. Construction and identification of recombinant plasmid LentiH1-shRNA
(A): Produce shRNA interference fragments sense and antisense oligo nucleotides were annealed to form complementary double strands DNA. M: DNA marker (DL 5000); 1-3: sh-MAT2A RNA; 4-6: sh-MAT2B RNA. (B): LentiH1 shuttle plasmid was double digested by Xho I and BamH I. 1-2: the product of LentiH1 shuttle plasmid was double digested; all released two fragments (7612 bp and 59 bp). M: DNA marker (DL 5000). (C): recombinant plasmids were identified by digesting. M: DNA marker (DL 5000). 1, 3, 5, 7, 9, 11: digested plasmid (as a control); 2, 4, 6, 8, 10, 12: undigested plasmid (2-6: sh-MAT2A; 8-12: sh-MAT2B)