慢病毒介导shRNA干扰MAT2A与MAT2B基因抑制猪肌内脂肪细胞分化

赵存真,易本驰,陈培荣,李建柱,赵云焕,朱忠珂

Lentivirus Mediated Interference Silencing MAT2A and MAT2B Inhibited Differentiation of Porcine Intramuscular Preadipocytes

CunZhen ZHAO,BenChi YI,PeiRong CHEN,JianZhu LI,YunHuan ZHAO,ZhongKe ZHU

表2 实时定量PCR扩增所用的引物序列

Table 2 Specific primers for real-time quantitative PCR

Gene 序列号Accession No. 序列Primer sequence (5′ to 3′) MAT2A NM_001167650.1 S: GTGGTTCGTGAAACCATTAAG A: ATCAGTGGCATAACCAAACAT MAT2B NM_001142832.1 S: TAGGAGCTGCTGTTTTGAGA A: CACACGCCATTTCATACTTG PPARγ NM_214379.1 S:AGGACTACCAAAGTGCCATCAAA A:GAGGCTTTATCCCCACAGACAC aP2 HM_453202 S:GAGCACCATAACCTTAGATGGA A:AAATTCTGGTAGCCGTGACA CEBP/α AF_103944.1 S: GCACTTGCAGTTCCAGATCG A:ACCCGGTACTCGTTGCTGTT β-actin NM_007393 S: GGACTTCGAGCAGGAGATGG A: AGGAAGGAGGGCTGGAAGAG