慢病毒介导shRNA干扰MAT2A与MAT2B基因抑制猪肌内脂肪细胞分化

赵存真,易本驰,陈培荣,李建柱,赵云焕,朱忠珂

Lentivirus Mediated Interference Silencing MAT2A and MAT2B Inhibited Differentiation of Porcine Intramuscular Preadipocytes

CunZhen ZHAO,BenChi YI,PeiRong CHEN,JianZhu LI,YunHuan ZHAO,ZhongKe ZHU

表1 猪MAT2A和MAT2B的shRNA序列参数

Table 1 Design of shRNA targeting on porcine MAT2A and MAT2B gene

RNAi 序列 Primer sequence (5′ to 3′) sh-MAT2A-1 sh-MAT2A-2 sh-MAT2A-3 sh-MAT2B-1 sh-MAT2B-2 sh-MAT2B-3 sh-scramble 5′-GATCCGCAGCAGTCACCAGATATTGCctcgagGCAATATCTGGTGACTGCTGCTTTTTC-3′ 5′-TCGAGAAAAAGCAGCAGTCACCAGATATTGCctcgagGCAATATCTGGTGACTGCTGCG-3′ 5′-GATCCGCACACAAGCTCAATGCCAAActcgagTTTGGCATTGAGCTTGTGTGCTTTTTC-3′ 5′-TCGAGAAAAAGCACACAAGCTCAATGCCAAActcgagTTTGGCATTGAGCTTGTGTGCG-3′ 5′-TCGAGAAAAAGCCAAGTGGCAGATTTGTTATctcgagATAACAAATCTGCCACTTGGCG-3′ 5′-GATCCGGAGAGTGCCGTGACTGTTATctcgagATAACAGTCACGGCACTCTCCTTTTTC-3′ 5′-GATCCGCACAGCGACGAGTACAAGActcgagTCTTGTACTCGTCGCTGTGCTTTTTC-3′ 5′-TCGAGAAAAAGCACAGCGACGAGTACAAGActcgagTCTTGTACTCGTCGCTGTGCG-3′ 5′-GATCCGGAGAGTGCCGTGACTGTTATctcgagATAACAGTCACGGCACTCTCCTTTTTC-3′ 5′-TCGAGAAAAAGGAGAGTGCCGTGACTGTTATctcgagATAACAGTCACGGCACTCTCCG -3′ 5′-GATCCGCGAACACCATTTCGAATTGGctcgagCCAATTCGAAATGGTGTTCGCTTTTTC-3′ 5′-TCGAGAAAAAGCGAACACCATTTCGAATTGGctcgagCCAATTCGAAATGGTGTTCGCG-3′ 5′-GATCCGACACCTACGCAAAACCCTctcgagGACACCTACGCAAAACCCTTTTTTC-3′ 5′-TCGAGAAAAAGACACCTACGCAAAACCCTctcgagGACACCTACGCAAAACCCTG-3′