斑翅果蝇气味结合蛋白OBP56h与小分子化合物的结合特征

李都,牛长缨,李峰奇,罗晨

Binding Characterization of Odorant Binding Protein OBP56h in Drosophila suzukii with Small Molecular Compounds

Du LI,ChangYing NIU,FengQi LI,Chen LUO

表1 候选配基与斑翅果蝇OBP56h蛋白的结合特性

Table 1 Binding affinities of DsuzOBP56h protein with candidate ligands

化学配基 Chemical ligand CAS number IC50 (μmol·L-1) 解离常数Ki Dissociation constant (μmol·L-1) 味觉物质 Tastant 苯甲地那铵Denatonium benzoate 3734-33-6 — — N-苯基硫脲N-Phenylthiourea 103-85-5 — — 盐酸小檗碱Berberine chloride 633-65-8 21.06 12.16 香豆素Coumarin 91-64-5 29.22 17.93 蔗糖Sucrose 57-50-1 — — 海藻糖Trehalose 6138-23-4 — — 黄酮类化合物Flavonoids 柚皮素Naringenin 67604-48-2 36.20 25.32 植物挥发物Plant volatiles 叶绿醇Phytol 7541-49-3 — — 乙偶姻Acetoin 513-86-0 — — 乙酸乙酯Ethyl acetate 141-78-6 — — β-环柠檬醛β-Cyclocitral 432-25-7 44.54 31.37 乙酸芳樟酯Linalyl acetate 115-95-7 — — 果蝇信息素 Drosophila pheromones 肉豆蔻酸甲酯Methyl myristate 124-10-7 — — 月桂酸甲酯Methyl laurate 111-82-0 — — 棕榈酸甲酯Methyl palmitate 112-39-0 — — 顺-9-二十三烯9Z-Tricosene 27519-02-4 — — 顺-7-二十三烯7Z-Tricosene 52078-42-9 — — 顺-11-十八碳烯酸乙酯11-cis-Vaccenyl acetate 6186-98-7 — —