福清山羊与努比亚黑山羊背最长肌比较转录组分析
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刘远,李文杨,吴贤锋,黄勤楼,高承芳,陈鑫珠,张晓佩
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Transcriptome Analysis of Differentially Gene Expression Associated with longissimus doris Tissue in Fuqing Goat and Nubian Black Goat
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Yuan LIU,WenYang LI,XianFeng WU,QinLou HUANG,ChengFang GAO,XinZhu CHEN,XiaoPei ZHANG
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表4 测序结果的qRT-PCR验证
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Table 4 Validation of sequencing results by qRT-PCR
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基因
Gene | 转录组测序RNA-Seq | 荧光定量PCR Real-time PCR | 表达倍数(福清山羊/努比亚黑山羊)
log2FC (Fuqing goats/Nubian Black goats) | 表达倍数(福清山羊/努比亚黑山羊)
log2FC (Fuqing goats/Nubian Black goats) | | TMSSF2 | -2.41±0.93 | -2.90±2.51 | | CD52 | 2.48±0.56 | 2.60±1.37 | | TASR1 | 3.43±2.61 | 1.80±0.86 | | GN-1377 | 2.51±1.20 | 0.98±0.76 | | GN-1378 | 2.73±0.33 | 1.08±0.41 | | GN-1272 | 1.22±1.00 | 1.07±0.68 | | MyHcⅠ | -0.24±1.04 | -0.11±1.36 | | MyHcⅡb | -0.62±0.39 | -0.51±1.22 |
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