整合微生物组菌剂的提出、研发与应用

刘波,陈倩倩,王阶平,阮传清,陈燕萍,夏江平,车建美,陈峥,潘志针,文笑,朱育菁,张海峰,郑雪芳

Proposition, Development and Application of the Integrated Microbiome Agent (IMA)

Bo LIU,QianQian CHEN,JiePing WANG,ChuanQing RUAN,YanPing CHEN,JiangPing XIA,JianMei CHE,Zheng CHEN,ZhiZhen PAN,Xiao WEN,YuJing ZHU,HaiFeng ZHANG,XueFang ZHENG

表2 整合微生物组菌剂产品样品芽孢杆菌种类鉴定

Table 2 Identification of Bacillus-like species in the product of the integrated microbiome agent

菌落编号 Colony number 菌株编号 Strain number 标准菌株学名 Name of Bacillus-like species 16S rDNA相似度 16S rDNA similarity (%) 1. FJAT-46225 [1] 芽孢杆菌Bacillus sp. 97.13 2. FJAT-46301 [2] 嗜气芽孢杆菌Bacillus aerophilus 28KT 100.00 3. FJAT-46217 100.00 4. FJAT-46253 [3] 高地芽孢杆菌Bacillus altitudinis 41KF2bT 100.00 5. FJAT-46318 [4] 解淀粉芽孢杆菌Bacillus amyloliquefaciens FZB42T 99.72 6. FJAT-46213 [5] 蜡样芽孢杆菌Bacillus cereus ATCC 14579T 99.70 7. FJAT-46201 99.64 8. FJAT-46307 [6] 阿氏芽孢杆菌Bacillus aryabhattai B8W22T 100.00 9. FJAT-46281 97.89 10. FJAT-46278 100.00 11. FJAT-46272 100.00 12. FJAT-46223 100.00 13. FJAT-46186 99.86 14. FJAT-46270 [7] 克劳氏芽孢杆菌Bacillus clausii DSM 8716T 99.50 15. FJAT-46194 99.20 菌落编号 Colony number 菌株编号 Strain number 标准菌株学名 Name of Bacillus-like species 16S rDNA相似度 16S rDNA similarity (%) 16. FJAT-46263 [8] 蚯蚓芽孢杆菌Bacillus eiseniae A1-2T 99.58 17. FJAT-46178 99.72 18. FJAT-46289 [9] 丝状芽孢杆菌Bacillus filamentosus SGD-14T 100.00 19. FJAT-46204 100.00 20. FJAT-46297 [10] 柯赫芽孢杆菌Bacillus kochii WCC 4582T 99.93 21. FJAT-46258 99.79 22. FJAT-46246 100.00 23. FJAT-46230 99.65 24. FJAT-46221 99.65 25. FJAT-46208 99.72 26. FJAT-46202 99.86 27. FJAT-46176 98.86 28. FJAT-46175 99.72 29. FJAT-46313 [11] 地衣芽孢杆菌Bacillus licheniformis ATCC 14580T 99.10 30. FJAT-46312 99.30 31. FJAT-46296 99.70 32. FJAT-46271 99.37 33. FJAT-46268 99.23 34. FJAT-46266 99.37 35. FJAT-46260 99.40 36. FJAT-46244 99.72 37. FJAT-46239 99.30 38. FJAT-46237 97.70 39. FJAT-46235 99.16 40. FJAT-46233 99.37 41. FJAT-46218 98.30 42. FJAT-46215 99.65 43. FJAT-46212 97.50 44. FJAT-46203 99.93 45. FJAT-46200 99.72 46. FJAT-46196 99.79 47. FJAT-46188 99.60 48. FJAT-46182 99.70 49. FJAT-46180 99.60 50. FJAT-46173 99.23 51. FJAT-46197 [12] 甲基营养型芽孢杆菌Bacillus methylotrophicus KACC 13105T 100.00 52. FJAT-46274 [13] 根际芽孢杆菌Bacillus rhizosphaerae SC-N012T 99.09 53. FJAT-46174 [14] 沙福芽孢杆菌Bacillus safensis FO-36bT 100.00 54. FJAT-46311 [15] 索诺拉沙漠芽孢杆菌Bacillus sonorensis NBRC 101234T 99.44 55. FJAT-46255 99.30 56. FJAT-46199 99.37 57. FJAT-46177 99.44 菌落编号 Colony number 菌株编号 Strain number 标准菌株学名 Name of Bacillus-like species 16S rDNA相似度 16S rDNA similarity (%) 58. FJAT-46316 [16] 枯草芽孢杆菌茵氏亚种Bacillus subtilis subsp. inaquosorum KCTC 13429T 99.72 59. FJAT-46298 99.93 60. FJAT-46295 99.93 61. FJAT-46279 99.93 62. FJAT-46259 99.93 63. FJAT-46252 99.79 64. FJAT-46251 99.72 65. FJAT-46248 99.93 66. FJAT-46247 99.93 67. FJAT-46242 99.79 68. FJAT-46238 99.93 69. FJAT-46231 99.93 70. FJAT-46229 99.93 71. FJAT-46220 99.37 72. FJAT-46211 99.93 73. FJAT-46206 99.93 74. FJAT-46185 99.79 75. FJAT-46184 99.93 76. FJAT-46262 [17] 枯草芽孢杆菌枯草亚种Bacillus subtilis subsp. subtilis NCIB 3610T 99.93 77. FJAT-46241 99.93 78. FJAT-46234 99.93 79. FJAT-46219 99.93 80. FJAT-46214 99.93 81. FJAT-46190 99.93 82. FJAT-46179 99.93 83. FJAT-46314 [18] 特基拉芽孢杆菌Bacillus tequilensis KCTC 13622T 100.00 84. FJAT-46257 100.00 85. FJAT-46250 99.79 86. FJAT-46216 100.00 87. FJAT-46191 99.86 88. FJAT-46249 [19] 人参哈格瓦氏菌Bhargavaea ginsengige 14T 99.79 89. FJAT-46227 99.93 90. FJAT-46224 99.93 91. FJAT-46192 99.79 92. FJAT-46276 [20] 赖氨酸芽孢杆菌Lysinibacillus sp. 97.76 93. FJAT-46275 [21] 淤泥大洋芽孢杆菌Oceanobacillus caeni S-11T 99.30 94. FJAT-46254 [22] 拾蛤鸟氨酸芽孢杆菌Ornithinibacillus scapharcae TW25T 98.47 95. FJAT-46303 [23] 盐反硝化枝芽孢杆菌Virgibacillus halodenitrificans DSM10037T 99.79 96. FJAT-46195 99.86 97. FJAT-46309 [24] 海洋枝芽孢杆菌Virgibacillus oceani MY11T 98.70 98. FJAT-46232 98.30 99. FJAT-46198 98.29 100. FJAT-46222 [25] 盐湖枝芽孢杆菌Virgibacillus salinus XH-22T 98.48