图2. 转VqSTS26、VqSTS32无核白的遗传转化过程与转基因植株的鉴定
A:基因克隆与过表达载体构建Gene cloning and vector construction。a:pCAMBIA35S:: VqSTSs:: GFP载体 pCAMBIA35S:: VqSTSs:: GFP vector;b:VqSTS26、VqSTS32目的基因扩增Gene amplification of VqSTS26 and VqSTS32;c:VqSTS26、VqSTS32基因连接表达载体pCAMBIA2300的双酶切检测,CK为pCAMBIA2300的空载体对照 Double enzyme digestion detection of recombinant plasmid, CK was the empty vector as control;d:VqSTS26、VqSTS32转化农杆菌GV3101的PCR检测,CK1、CK2分别为每个基因重组质粒的阳性对照 PCR detection of agrobacterium GV3101 transformed with VqSTS26 and VqSTS32. CK1 and CK2 were positive controls of recombinant plasmid for each gene。M:Trans 2k plus DNA maker。B:无核白分生愈伤组织的诱导（a—d）、遗传转化（e—h）与转化后的诱导成苗、扩繁与移栽炼苗（i—n） Induction (a-d) and genetic transformation (e-h) of Thompson Seedless meristem callus and induction into seedlings after transformation (i-n)。C:无核白葡萄转VqSTS26、VqSTS32抗性株系的PCR检测和Western blot检测PCR amplification and Western blot detection of Thompson Seedless transgenic lines with VqSTS26 and VqSTS32

Fig. 2. Transformation and identification of transgenic VqSTS26 and VqSTS32 Thompson Seedless mutant plants