玉米花期根系结构的表型变异与全基因组关联分析

张小琼,郭剑,代书桃,任元,李凤艳,刘京宝,李永祥,张登峰,石云素,宋燕春,黎裕,王天宇,邹华文,李春辉

Phenotypic Variation and Genome-wide Association Analysis of Root Architecture at Maize Flowering Stage

XiaoQiong ZHANG,Jian GUO,ShuTao DAI,Yuan REN,FengYan LI,JingBao LIU,YongXiang LI,DengFeng ZHANG,YunSu SHI,YanChun SONG,Yu LI,TianYu WANG,HuaWen ZOU,ChunHui LI

表4 影响玉米地下节根相关性状的SNP及最可能的候选基因及其功能注释

Table 4 List of significant SNPs affecting maize belowground nodal root-related traits and the plausible candidate genes and their functional annotations

性状 Trait SNP Chr. 位置a Position (bp) P值 P value 候选基因b Candidate gene 基因功能注释 Gene annotation 参考文献c Reference RLN Chr.1.S_31312069 1 31312069 3.07E-06 Zm00001d028337 富含脯氨酸的受体样蛋白激酶PERK4 Proline-rich receptor-like protein kinase PERK4 RLN Chr.1.S_31312087 1 31312087 3.07E-06 Zm00001d028344 推定的金属耐受蛋白C3 Putative metal tolerance protein C3 RDW Chr.3.S_103120493 3 103120493 1.07E-05 Zm00001d041183 蛋白激酶超家族蛋白质/苏氨酸蛋白激酶prpf4B-liken Protein kinase superfamily proteiserine/threonine-protein kinase prpf4B-liken RV Chr.3.S_104251070 3 104251070 8.33E-06 Zm00001d041192 蔗糖转运蛋白4 Sucrose transporter4 RDW Chr.4.S_9975093 4 9975093 7.24E-06 Zm00001d048943 硫氧还蛋白超家族蛋白 Thioredoxin superfamily protein [14] RV Chr.4.S_38214896 4 38214896 3.38E-06 Zm00001d049623 SNARE相关蛋白 SNARE-associated protein-related Zm00001d049627 蛋氨酸氨肽酶 Methionine aminopeptidase RV Chr.4.S_63797065 4 63797065 7.79E-06 Zm00001d050069 海藻糖-6-磷酸合成酶8 Trehalose-6-phosphate synthase8 RV Chr.4.S_100804613 4 100804613 8.30E-06 Zm00001d050558 OSJNBa0043A12.20蛋白 OSJNBa0043A12.20 protein TRN Chr.4.S_246664102 4 246664102 3.96E-06 Zm00001d054104 E3泛素蛋白连接酶UPL3 E3 ubiquitin-protein ligase UPL3 Zm00001d054105 尿苷激酶样蛋白3 Uridine kinase-like protein 3 RDW Chr.6.S_34950886 6 34950886 9.19E-06 Zm00001d035587 G型凝集素S受体样丝氨酸/苏氨酸蛋白激酶At2g19130 G-type lectin S-receptor-like serine/threonine-protein kinase At2g19130 [5,20] Zm00001d035588 G型凝集素S受体样丝氨酸/苏氨酸蛋白激酶At2g19130 G-type lectin S-receptor-like serine/threonine-protein kinase At2g19130 RDW Chr.6.S_108781517 6 108781517 6.09E-07 Zm00001d037004 Ras相关蛋白RABA1d Ras-related protein RABA1d [17] Zm00001d037010 毛发相关蛋白激酶iota Shaggy-related protein kinase iota RLN/TRN Chr.6.S_123079904 6 123079904 4.00E-06 Zm00001d037360 推定的bZIP转录因子超家族蛋白 Putative bZIP transcription factor superfamily protein [20] RLN/TRN Chr.6.S_123079927 6 123079927 4.00E-06 Zm00001d037368 未知 Unknown [20] RLN Chr.6.S_123845438 6 123845438 2.51E-06 Zm00001d037384 花青素3-O-葡糖基转移酶 Anthocyanidin 3-O-glucosyltransferase [20] TRN Chr.6.S_135051799 6 135051799 6.13E-06 Zm00001d037712 MAR结合丝状蛋白1 MAR-binding filament-like protein 1 RV Chr.6.S_136591446 6 136591446 6.19E-06 Zm00001d037751 晚期胚胎发育丰富（LEA）富含羟脯氨酸的糖蛋白家族 Late embryogenesis abundant (LEA) hydroxyproline-rich glycoprotein family RDW Chr.7.S_48142656 7 48142656 3.42E-06 Zm00001d019648 核酸结合蛋白1 Nucleic acid binding protein 1 [14] RDW Chr.7.S_101804639 7 101804639 7.71E-07 Zm00001d020242 锌指CCCH结构域的蛋白质64 Zinc finger CCCH domain-containing protein 64 [14] RLN Chr.8.S_55681954 8 55681954 5.47E-06 Zm00001d009324 AMSH样泛素蛋白硫酯酶3 AMSH-like ubiquitin thioesterase 3 RV Chr.8.S_100196991 8 100196991 1.68E-06 Zm00001d010119 冷休克蛋白CS66 Cold shock protein CS66 [20] RV Chr.8.S_100680141 8 100680141 1.07E-07 Zm00001d010128 酸性核糖体蛋白P2b（rpp2b） Acidic ribosomal protein P2b (rpp2b) [20] RV Chr.9.S_67485696 9 67485696 3.82E-07 Zm00001d046152 推定的RING-H2指蛋白ATL71 Putative RING-H2 finger protein ATL71 RV Chr.9.S_111860806 9 111860806 9.44E-06 Zm00001d046942 果糖-1,6-二磷酸酶 Fructose-1,6-bisphosphatase RV Chr.10.S_150110617 10 150110617 1.08E-05 Zm00001d026687 生长素响应因子17 Auxin response factor 17 [14] Zm00001d026694 生长素响应因子13 Auxin response factor 13