山羊Kruppel样转录因子家族在前体脂肪细胞分化中的表达模式及相关性分析

朱江江,林亚秋,王永,林森

Expression Profile and Correlations of Kruppel Like Factors During Caprine (Capra Hircus) Preadipocyte Differentiation

JiangJiang ZHU,YaQiu LIN,Yong WANG,Sen LIN

表1 引物信息

Table 1 Primers information

目的基因 Gene 登录号 Accession No. 引物序列（5′-3′） Primer sequences 退火温度 TM (℃) 产物长度 Products length (bp) KLF2 KU041748 S: GCGGCAAGACCTACACCAA 60 144 A: TGTGCTTGCGGTAGTGGC KLF3 KU041753 S: CTCGGTGTCATACCCGTCTAAT 60 128 A: CCATCTGCATCCCGTGAGAC KLF4 KU041754 S: GTCGGTCATCAGTGTTAGCAAAGG 62 126 A: ACGGTGCACGAGGAGACAGTCT KLF5 KU041751 S:CACCTCCATCCTATGCTGCTAC 62 169 A:CAGCCTGGGTAATCGCAGTAGT KLF6 KU041749 S:GCCTCTGAGATCAAATTCGACA 62 189 A:GGAGGACTCGCTGCTCACAT KLF7 KU041750 S:TTCGGTGAGGACTTGGACTGTT 60 148 A:TGTCCCGAGAGAGCAGAATGTC KLF8 KX247671 S:GACTACAGCAAGAACCAGCAGC 62 153 A:CTCCTGTATGGATTCTGCGGT KLF9 KX247670 S:GGATACCTGGAAGGATTACTGC 60 172 A:GGAAGGACTCGACCCAGATT KLF10 KU643198 S:GTCAGATACTGCCAAGCCTCAC 62 133 A:GTTACACAGTTGGGCATCAGC KLF12 KX247669 S:GGTCTCCAAACGTCCACAAGTA 62 169 A:GAAACGGCTGTAGGGGATGTC KLF15 KU041752 S:CTGACTCGCAAACCCTGTGT 62 192 A:ACTCGGGGAAGCTGAAATG GAPDH XM_005680968 S:GCAAGTTCCACGGCACAG 62 118 A:TCAGCACCAGCATCACCC