马铃薯低温响应的ScmiR390-5p及其靶基因表达与结构分析

谢洁,王明,丁红映,李青,王万兴,熊兴耀,秦玉芝

Expression and Structural Analysis of SC MI390-5p and Its Target Genes in Potato Response to Low Temperature

Jie XIE,Ming WANG,HongYing DING,Qing LI,WanXing WANG,XingYao XIONG,YuZhi QIN

表1 本研究所用引物序列

Table 1 Primers used in this study

引物 Primer 序列（5′→3′） Sequence (5′→3′) 用途 Use SCLRRK1 F ATGACACTTTTGAGGTTTCTGTTAA SCLRRK1 CDS片段克隆 SCLRRK1 R TTAGCTTTCCGTGATTTCTTGA Clone of SCLRRK1 CDS fragments SCLRRK1 F1 GTTTTCTTCATTTCTTGATATGGC SCLRRK1DNA全长扩增 SCLRRK1 R1 GTTGAATAGTAGCATTCCCTTAATC Amplification of Full-length DNA of SCLRRK1 SCLRRK1 F2 GCTTGAGATCCTAACCACCCG SCLRRK1实时定量PCR SCLRRK1 R2 TCTTGCAGCATTTCTACCACCTT Quantitative real-time PCR of SCLRRK1 SCLRRK1 outer F3 GCTGATGGCGATGAATGAATGAACACTG RLM-5′RACE 分析作用位点 SCLRRK1 outer R3 CAACAAAGACGTTTCCCGCCAGT Rapid amplification of cDNA 5′ ends SCLRRK1 inner F4 CGCGGATCCGAACACTGCGTTTGCTGGCTTTGATG RLM-5′RACE 分析作用位点 SCLRRK1 inner R4 CCTGAGGCAATTTTCCGTGGAGA Rapid amplification of cDNA 5′ ends ScmiR390-5p CCAGCGTGAAGCUCAGGAGGG ScmiR390-5p实时荧光定量PCR ScmiR390-5p CCAGCGTGCGCUAUCCAUCUU Quantitative real-time PCR of ScmiR390-5p