基于小RNA深度测序技术鉴定侵染广东辣椒的病毒种类

汤亚飞,裴凡,李正刚,佘小漫,于琳,蓝国兵,邓铭光,何自福

Identification of Viruses Infecting Peppers in Guangdong by Small RNA Deep Sequencing

YaFei TANG,Fan PEI,ZhengGang LI,XiaoMan SHE,Lin YU,GuoBing LAN,MingGuang DENG,ZiFu HE

表4 用于检测14种病毒的引物

Table 4 Primers used to detect 14 viruses in this study

病毒Virus 引物Primer 序列Sequence (5′-3′) 目的片段Product (bp) ChiVMV ChiVMV1-F CGAGTCTCATAAGTAATTGCAC 752 ChiVMV1-R AAGACATTTACTGATACCCTCC TMGMV TMGMV1-F CCTCCCTGGTAATAGTACTATAC 407 TMGMV1-R CCAACTGTCCAATAGTACTTCT ChiRSV ChiRSV2-F GAGTTCCACAGAGTAATGATCT 500 ChiRSV2-R GACTAACTGACTCTCACTCTTAC BBWV-2 BBWV-2-1-F GAGTTCAGTTACAGAAATTGGG 674 BBWV-2-1-R CATATGGGTTCCTTGAAATGAC BPEV BPEV1-F AGGAGATGTGATTTCAGTAGAC 558 BPEV1-R TCTAATATTCTCTCCCCTCCTAG PeVYV-1 PeVYV1-1-F CTAGTCTACCTTAAGATCTCAGC 678 PeVYV1-1-R GTACTACCTTCTACCTATTTCGG PVMV PVMV2-F GGTTTGGTGTATAGAAAATGGG 758 PVMV2-R CAGACTCTATCAGGTGGTATAAC PeVYV-6 PeVYV6-1-F GAAAAGAAAGTGGTGGAAGTAG 794 PeVYV6-1-R GGCTACCAATTGATCTACTAGAG CaCV CaCV1-F CAGCTCTTAAATCAGTACTAGGG 472 CaCV1-R GTGTTTCCTGCATTAGTTCTAG PCV1 PCV1-F CAACACATATTCAGCATACTCC 608 PCV1-R GGGCGTATCTCTGATAATGATAG PMMoV PMMoV2-F GCAGATCCCCTACGGTTCAA 761 PMMoV2-R CGCTATTCATCATCGCCAAAGT PVY PVY2-F GAAGCGCGAGGAGAGAGAAA 728 PVY2-R TTATCGCTGCAACTCTGCCA CMV CMV-F ATGGACAAATCTGRATCWMCC 764 CMV-R CTGGATGGACAACCCGTTC TMV TMV-F CGGTCAGTGCCGAACAAGAA 600 TMV-R ATTTAAGTGGASGGAAAAVCACT