基于小RNA深度测序技术鉴定侵染广东辣椒的病毒种类

汤亚飞,裴凡,李正刚,佘小漫,于琳,蓝国兵,邓铭光,何自福

Identification of Viruses Infecting Peppers in Guangdong by Small RNA Deep Sequencing

YaFei TANG,Fan PEI,ZhengGang LI,XiaoMan SHE,Lin YU,GuoBing LAN,MingGuang DENG,ZiFu HE

表3 小RNA深度测序分析结果与RT-PCR验证结果比较

Table 3 Comparison of results between small RNA deep sequencing and RT-PCR

病毒 Virus	茂名-1 Maoming-1		茂名-2 Maoming-2		茂名-3 Maoming-3		茂名-4 Maoming-4		梅州 Meizhou		韶关-1 Shaoguan-1		韶关-2 Shaoguan-2
病毒 Virus	sRNA	RT-PCR	sRNA	RT-PCR	sRNA	RT-PCR	sRNA	RT-PCR	sRNA	RT-PCR	sRNA	RT-PCR	sRNA	RT-PCR
ChiVMV	+	+	+	+	+	+	-	-	-	+	-	-	-	-
TMGMV	-	-	-	-	+	+	+	+	-	-	-	+	-	-
BBWV-2	+	+	+	+	+	+	-	-	-	-	-	-	-	-
PCV1	+	+	-	-	-	-	+	+	-	-	+	+	+	+
CaCV	-	-	-	-	+	+	-	-	+	+	+	+	+	+
ChiRSV	+	+	+	+	+	+	-	-	-	-	-	-	-	-
PVMV	+	+	-	-	-	-	-	-	+	+	-	+	-	+
PMMoV	-	-	-	-	-	-	-	-	+	+	+	+	-	+
PVY	-	+	-	-	-	-	-	-	+	+	-	+	-	-
TMV	-	-	-	-	-	-	-	-	-	-	-	+	-	+
CMV	+	+	+	+	+	+	+	+	-	-	-	+	-	+
PeVYV-6	+	+	+	+	+	+	-	-	-	+	-	-	-	+
BPEV	+	+	+	+	+	+	-	-	+	+	-	+	-	-
PeVYV-1	+	+	+	+	+	+	-	-	-	+	-	+	-	+