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GST pull-down联合LC-MS/MS筛选柑橘抗溃疡病转录因子CsBZIP40的互作蛋白
窦万福,祁静静,胡安华,陈善春,彭爱红,许兰珍,雷天刚,姚利晓,何永睿(),李强()
Screening of Interacting Proteins of Anti-Canker Transcription Factor CsBZIP40 in Citrus by GST Pull-Down Combined with LC-MS/MS
DOU WanFu,QI JingJing,HU AnHua,CHEN ShanChun,PENG AiHong,XU LanZhen,LEI TianGang,YAO LiXiao,HE YongRui(),LI Qiang()

图3. 融合蛋白诱导表达、纯化检测
A：GST-CsBZIP40融合蛋白表达 Expression of GST-CsBZIP40;M1：Marker;1：无IPTG诱导表达对照Expression of GST-CsBZIP40 without IPTG;2：15℃加IPTG诱导表达IPTG and 15℃ induced expression。B：诱饵蛋白纯化Bait protein purification;3：全菌破碎离心后上清Supernatant after centrifugation;4：上清同GST孵育后流出液Supernatant after incubation with GST;M2：Marker;5、6：40 HYPERLINK "https://www.baidu.com/s?wd=mm&tn=SE_PcZhidaonwhc_ngpagmjz&rsv_dl=gh_pc_zhidao" \t "_blank" mmol?L-1洗脱GST-CsBZIP40 40 HYPERLINK "https://www.baidu.com/s?wd=mm&tn=SE_PcZhidaonwhc_ngpagmjz&rsv_dl=gh_pc_zhidao" \t "_blank" mmol?L-1 eluent GST-CsBZIP40。C：诱饵蛋白纯化后浓度检测Concentration detection after purification of bait protein;M3：SDS-PAGE Marker;7：标准BSA Standard BSA;8：GST-CsBZIP40。D：诱饵蛋白纯化后纯度检测Fineness detection after purification of bait protein;M4：WB Marker;9：GST-CsBZIP40

Fig. 3. Induced expression and purification detection of fusion protein