GST pull-down联合LC-MS/MS筛选柑橘抗溃疡病转录因子CsBZIP40的互作蛋白
窦万福,祁静静,胡安华,陈善春,彭爱红,许兰珍,雷天刚,姚利晓,何永睿(
),李强()
),李强()
Screening of Interacting Proteins of Anti-Canker Transcription Factor CsBZIP40 in Citrus by GST Pull-Down Combined with LC-MS/MS
DOU WanFu,QI JingJing,HU AnHua,CHEN ShanChun,PENG AiHong,XU LanZhen,LEI TianGang,YAO LiXiao,HE YongRui(),LI Qiang()
),LI Qiang()
图2. 融合表达载体构建。
A：CsBZIP40的PCR扩增 PCR amplification of CsBZIP40;M：2000 bp分子量标记2000 bp molecular marker;1：扩增产物,约1 530 bp Amplified product, about 1 530 bp。B：CsBZIP40-GST融合表达载体的构建 Construction of CsBZIP40-GST fusion expression vector。C：融合表达载体的酶切验证Enzymic digestion of CsBZIP40-GST vector;1：完整质粒条带The complete plasmid band;2：CsBZIP40-GST;M：10 000 bp分子量标记10 000 bp molecular marker
Fig. 2. Construction of fusion expression vector
