GST pull-down联合LC-MS/MS筛选柑橘抗溃疡病转录因子CsBZIP40的互作蛋白
窦万福,祁静静,胡安华,陈善春,彭爱红,许兰珍,雷天刚,姚利晓,何永睿(
),李强()
),李强()
Screening of Interacting Proteins of Anti-Canker Transcription Factor CsBZIP40 in Citrus by GST Pull-Down Combined with LC-MS/MS
DOU WanFu,QI JingJing,HU AnHua,CHEN ShanChun,PENG AiHong,XU LanZhen,LEI TianGang,YAO LiXiao,HE YongRui(),LI Qiang()
),LI Qiang()
图1. 过表达CsBZIP40柑橘植株验证和溃疡病抗性评价
A：过表达CsBZIP40转基因植株表型 Phenotypes of CsBZIP40 overexpression lines。B：转基因植株系中CsBZIP40的阳性检测The positive detection of CsBZIP40 in transgenic lines;M：Marker;1：阳性检测条带Positive detection band。C：过表达CsBZIP40的相对表达量 The relative expression of overexpressed CsBZIP40。D：接种溃疡病菌的转基因植株和野生型对照叶片 Disease spots of transgenic lines and the wild-type (WT) inoculated with LB and Xcc。E：接种溃疡病菌的转基因植株和野生型对照病斑面积 Lesion area of transgenic lines and the wild-type inoculated with Xcc;F：接种溃疡病菌的转基因植株和野生型对照病情指数 Disease index of transgenic lines and the wild-type inoculated with Xcc
Fig. 1. Verification and canker resistance evaluation of overexpressed CsBZIP40 lines
