p66Shc在绵羊卵母细胞中的表达及其与胞质氧化还原稳态的关系
张通1,2,栗瑞兰1,2,范晓梅1,3,刘春洁1,海日汗1,霍敏1,张家新1(
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Expression of p66Shc and Its Relationship with Cytoplasmic Redox Homeostasis in Sheep Oocytes
ZHANG Tong1,2,LI RuiLan1,2,FAN XiaoMei1,3,LIU ChunJie1,HAI RiHan1,HUO Min1,ZHANG JiaXin1()
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图6. 过氧化氢诱导的氧化应激上调 p66Shc的表达
A：ROS 水平;B：FAD++/NAD(P)H 比率;C1、C2：对照组（30 min 和1 h）;C3、C4：处理组（100 μM H2O2 分别处理30 min 和1 h）; 绿色标记p66Shc,蓝色标记细胞核,白色箭头指向细胞核,比例尺 表示30 μm。不同字母表示差异显著(P<0.05), 相同字母表示差异不 显著(P>0.05)
Fig. 6. Hydrogen peroxide induced oxidative stress upregulated the expression of p66Shc
A: ROS level; B: FAD ++ / NAD (P) H fluorescence ratio; C1、C2: Control group (30 min or 1 h); C3、C4: treatment group (100 μM H2O2 treatment 30 min or 1 h, respectively); Green labeled p66Shc, blue labeled nuclear, white arrows point to the nucleus, The scale bar represents 30 μm. The different superscripts mean significant difference (P<0.05), and the same superscripts mean no significant difference (P>0.05)
