p66Shc在绵羊卵母细胞中的表达及其与胞质氧化还原稳态的关系

张通,栗瑞兰,范晓梅,刘春洁,海日汗,霍敏,张家新

Expression of p66Shc and Its Relationship with Cytoplasmic Redox Homeostasis in Sheep Oocytes

Tong ZHANG,RuiLan LI,XiaoMei FAN,ChunJie LIU,RiHan HAI,Min HUO,JiaXin ZHANG

表1 p66Shc和β-actin基因引物序列

Table 1 The oligonucleotide primer for p66Shc and β-actin

基因 Gene 基因ID Gene ID 退火温度 Annealing temperature (℃) 引物序列（5'-3'） The sequence of primer 产物长度 Product length (bp) p66Shc 101113548 60 F: CGGGGTTTCCTACTTGGTT R: ACGGCTACAGGGCTTTCTC 169 β-actin 443052 60 F: GTCATCACCATCGGCAATGA R: CGTGAATGCCGCAGGATT 88