基于转录-代谢联合分析哈茨木霉ACCC32527 对NaCl胁迫的分子调节

向杰,陈敬师,夏鑫鑫,刘快,李世贵,顾金刚

Molecular Regulation of Trichoderma harzianum ACCC32527 Response to NaCl Based on Transcriptome and Metabolome Analysis

Jie XIANG,JingShi CHEN,XinXin XIA,Kuai LIU,ShiGui LI,JinGang GU

表1 RT-qPCR验证基因及引物设计

Table 1 Gene selection and primer design for RT-qPCR

基因ID Gene ID 引物序列Primer sequence (5′→3′) c55075.graph_c0 F-5′ ATTCCCGTCTTAGGGTTCG 3′; R-5′ TGTTGGCTGCTACGCTTT 3′ c56173.graph_c0 F-5′ CCAGCCTTCCTTCTCCTC 3′; R-5′ ATGGTGCCACAGTCTATTCC 3′ c44870.graph_c0 F-5′ TGCTGAAGCGTGAGGTTG 3′; R-5′ AAGCCCGAGTCTTGTTGC 3′ c57043.graph_c0 F-5′ ATTGGCAGGTAGAGTCGT 3′; R-5′ TCATTGATAAACTGGTCCTT 3′ c60715.graph_c3 F-5′ GCGGGAAATCTGCCTTAG 3′; R-5′ GCTGGGTGACGCATAGCT 3′ c60689.graph_c1 F-5′ TACGACCAAACGAGTGCG 3′; R-5' GGCGTCAAGCAGGTGTCT 3′ c55738.graph_c0 F-5′ CTGCCGATTGTTCCGTAT 3′; R-5' GCTTATTAGCCAAGTTGTTTCT 3′ UCE F-5′ GTGGCGGCAGCACTTGTTAT 3′; R-5'ATGACGAACGAAAAGCACCG 3′