蔬菜土传病原菌三重PCR检测体系的建立与应用
刘芮池1,2,程有普2,柴阿丽1(
),石延霞1,谢学文1,帕提古丽3,李宝聚1()
),石延霞1,谢学文1,帕提古丽3,李宝聚1()
Establishment and Application of a Triplex PCR Detection System for Vegetable Soil-Borne Pathogens
LIU RuiChi1,2,CHENG YouPu2,CHAI ALi1(),SHI YanXia1,XIE XueWen1, PATIGULI3,LI BaoJu1()
),SHI YanXia1,XIE XueWen1, PATIGULI3,LI BaoJu1()
图6. 模拟带菌基质三重PCR检测灵敏度
M：DL 5000 Marker;1—7：尖镰孢和大丽轮枝菌浓度分别为108、107、106、105、104、103、102个孢子/g,瓜果腐霉浓度分别为10、1、10-1、10-2、10-3、10-4、10-5 mg·g-1 The concentration of F. oxysporum and V. dahliae is 108, 107, 106, 105, 104, 103, 102 spores/g, respectively. The concentration of P. aphanidermatum is 10, 1, 10-1, 10-2, 10-3, 10-4, 10-5 mg·g-1, respectively;8：未加菌基质No pathogen in soil;9：阴性对照Negative control
Fig. 6. Sensitivity of triplex PCR detection in artificially inoculated substrate
