蔬菜土传病原菌三重PCR检测体系的建立与应用
刘芮池1,2,程有普2,柴阿丽1(
),石延霞1,谢学文1,帕提古丽3,李宝聚1()
),石延霞1,谢学文1,帕提古丽3,李宝聚1()
Establishment and Application of a Triplex PCR Detection System for Vegetable Soil-Borne Pathogens
LIU RuiChi1,2,CHENG YouPu2,CHAI ALi1(),SHI YanXia1,XIE XueWen1, PATIGULI3,LI BaoJu1()
),SHI YanXia1,XIE XueWen1, PATIGULI3,LI BaoJu1()
图2. 三重PCR特异性检测
M：DL 5000 Marker;1：瓜果腐霉、尖镰孢、大丽轮枝菌P. aphanidermatum, F. oxysporum and V. dahliae;2、3：茄镰孢F. solani;4、5：辣椒疫霉P. capsici;6、7：核盘菌S. sclerotiorum;8、9：立枯丝核菌R. solani;10、11：灰葡萄孢B. cinerea;12、13：茄科劳尔氏菌R. solanacearum;14、15：胡萝卜果胶杆菌P. carotovorum;16、17：密执安棒形杆菌密执安亚种C. michiganensis subsp. michiganensis;18：阴性对照Negative control
Fig. 2. The specific detection of triplex PCR
