蔬菜土传病原菌三重PCR检测体系的建立与应用

刘芮池,程有普,柴阿丽,石延霞,谢学文,帕提古丽,李宝聚

Establishment and Application of a Triplex PCR Detection System for Vegetable Soil-Borne Pathogens

RuiChi LIU,YouPu CHENG,ALi CHAI,YanXia SHI,XueWen XIE,PATIGULI ,BaoJu LI

表4 不同地区病害样本三重PCR检测

Table 4 Identification of diseases samples in different areas by triplex PCR

采样时间 Sampling time	采样地点 Sampling site	寄主 Host	疑似病害 Suspected disease	采样部位 Sampling position	三重PCR Triple PCR	分离培养 Isolated culture	样本数量 Number of samples
2017-12-09	浙江苍南 Cangnan, Zhejiang	茄子Eggplant	黄萎病Verticillium wilt	茎基Stem	+	V. dahliae	4
				土壤Soil	+	V. dahliae	4
2018-01-16	山东寿光 Shouguang, Shandong	黄瓜Cucumber	枯萎病Fusarium wilt	茎基Stem	+	F. oxysporum	1
				土壤Soil	+	F. oxysporum	1
2018-03-15	浙江苍南 Cangnan, Zhejiang	黄瓜Cucumber	枯萎病Fusarium wilt	茎基Stem	+	F. oxysporum	1
				土壤Soil	+	F. oxysporum	1
2018-03-20	山东寿光 Shouguang, Shandong	黄瓜Cucumber	根腐病Root rot	茎基Stem	+	P. aphanidermatum	2
				土壤Soil	+	P. aphanidermatum	2
2018	浙江苍南 Cangnan, Zhejiang	黄瓜Cucumber	枯萎病Fusarium wilt	茎基Stem	+	F. oxysporum	2
				土壤Soil	+	F. oxysporum	2
2018-10-21	山东寿光 Shouguang, Shandong	黄瓜Cucumber	根腐病Root rot	茎基Stem	+	P. aphanidermatum	1
				土壤Soil	+	P. aphanidermatum	1
2018-10-21	山东寿光 Shouguang, Shandong	黄瓜Cucumber	根腐病Root rot	茎基Stem	-	—	1
				土壤Soil	-	—	1
2018-03-15	浙江苍南 Cangnan, Zhejiang	黄瓜Cucumber	枯萎病Fusarium wilt	茎基Stem	-	—	1
				土壤Soil	-	—	1
2018-03-20	山东寿光 Shouguang, Shandong	黄瓜Cucumber	根腐病Root rot	茎基Stem	-	—	1
				土壤Soil	-	—	1
2018-08-31	新疆喀什 Kashi, Xinjiang	甜瓜Muskmelon	枯萎病Fusarium wilt	茎基Stem		F. oxysporum	11
				茎基Stem	-	—	4
2019-01-02	辽宁绥中 Suizhong, Liaoning	黄瓜Cucumber	枯萎病Fusarium wilt	茎基Stem	+	F. oxysporum	3
				茎基Stem	-	—	3
2017-2018	浙江苍南 Cangnan, Zhejiang	—	—	土壤Soil	-	—	48
				土壤Soil	+	F. oxysporum	12
				土壤Soil	+	V. dahliae	12
2018	山东寿光 Shouguang, Shandong	—	—	土壤Soil	-	—	27
				土壤Soil	+	V. dahliae	3
				土壤Soil	+	F. oxysporum	6
				土壤Soil	+	P. aphanidermatum	12
2018-08-24	宁夏平罗 Pingluo, Ningxia	—	—	土壤Soil	+	P. aphanidermatum	9
2018-08-24	浙江永康 Yongkang, Zhejiang	—	—	土壤Soil	+	P. aphanidermatum	6