蔬菜土传病原菌三重PCR检测体系的建立与应用
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刘芮池,程有普,柴阿丽,石延霞,谢学文,帕提古丽,李宝聚
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Establishment and Application of a Triplex PCR Detection System for Vegetable Soil-Borne Pathogens
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RuiChi LIU,YouPu CHENG,ALi CHAI,YanXia SHI,XueWen XIE,PATIGULI ,BaoJu LI
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表2 供试引物
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Table 2 The primers used in the test
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| 病原菌Pathogen | 序列Sequence (5′-3′) | 产物大小Product length (bp) | 参考文献Reference | 瓜果腐霉
P. aphanidermatum | AsAPH2B: GCGCGTTGTTCACAATAAATTGC | 163 | [19] | | AsPyF: CTGTTCTTTCCTTGAGGTG | | 尖镰孢F. oxysporum | FOF1: ACATACCACTTGTTGCCTCG | 328 | [20] | | FOR1: CGCCAATCAATTTGAGGAACG | | 大丽轮枝菌V. dahliae | VActF: TAATTCACAATGGAGGGTAGG | 530 | [21] | | VActR: GTAAGGATACCACGCTTGG |
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