蔬菜土传病原菌三重PCR检测体系的建立与应用
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刘芮池,程有普,柴阿丽,石延霞,谢学文,帕提古丽,李宝聚
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Establishment and Application of a Triplex PCR Detection System for Vegetable Soil-Borne Pathogens
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RuiChi LIU,YouPu CHENG,ALi CHAI,YanXia SHI,XueWen XIE,PATIGULI ,BaoJu LI
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表1 供试菌株
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Table 1 The strains used in the test
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病原菌
Pathogen | 寄主
Host | 菌株编号
Strain number | 引物 Primer | | AsAPH2B/AsPyF | FOF1/FOR1 | VActF/VActR | | 瓜果腐霉P. aphanidermatum | 番茄Tomato | FQ15092209 | + | - | - | | 尖镰孢F. oxysporum | 黄瓜Cucumber | HG12090201 | - | + | - | | 大丽轮枝菌V. dahliae | 茄子Eggplant | QZ12061103 | - | - | + | | 茄镰孢F. solani | 黄瓜Cucumber | HG14060601 | - | - | - | | 芹菜Celery | QC15061008 | - | - | - | | 核盘菌Sclerotinia sclerotiorum | 青花菜Broccoli | QHC18032301 | - | - | - | | 茄子Eggplant | QZ11102201 | - | - | - | | 辣椒疫霉Phytophthora capsici | 辣椒Pepper | LJ12010805 | - | - | - | | 辣椒Pepper | LJ18081001 | - | - | - | | 灰葡萄孢Botrytis cinerea | 番茄Tomato | FQ11051301 | - | - | - | | 黄瓜Cucumber | HG11021509 | - | - | - | | 立枯丝核菌Rhizoctonia solani | 白菜Chinese cabbage | BC16052001 | - | - | - | | 生菜Lettuce | SC16052001 | - | - | - | | 茄科劳尔氏菌Ralstonia solanacearum | 番茄Tomato | FQ12080802 | - | - | - | | 番茄Tomato | FQ16060706 | - | - | - | | 胡萝卜果胶杆菌Pectobacterterium carotovorum | 黄瓜Cucumber | HG1501503604 | - | - | - | | 白菜Chinese cabbage | BC18082306 | - | - | - | 密执安棒形杆菌密执安亚种
Clavibacter michiganensis subsp. michiganensis | 番茄Tomato | FQ18082408 | - | - | - | | 番茄Tomato | FQ18082410 | - | - | - |
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