水稻转录因子OsWRKY68蛋白质的表达特征及其功能特性
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陈悦,王田幸子,杨烁,张彤,马金姣,燕高伟,刘玉晴,周艳,史佳楠,兰金苹,魏健,窦世娟,刘丽娟,杨明,李莉云,刘国振(  )
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Expression Profiling and Functional Characterization of Rice Transcription Factor OsWRKY68
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Yue CHEN,TianXingZi WANG,Shuo YANG,Tong ZHANG,JinJiao MA,GaoWei YAN,YuQing LIU,Yan ZHOU,JiaNan SHI,JinPing LAN,Jian WEI,ShiJuan DOU,LiJuan LIU,Ming YANG,LiYun LI,GuoZhen LIU(  )
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图1. OsWRKY68蛋白质在水稻不同生长发育时期和不同组织的表达特征 WB条件:上样量10 μL,电泳条件:80 V,20 min;160 V,90 min,胶浓度10% tricine,电泳槽Mini PROTEAN Tetra cell,转膜条件:100 V,60 min,膜PVDF,一抗:抗OsWRKY68抗体,抗OsHSP82抗体,二抗:羊抗兔二抗,羊抗鼠二抗。HSP:水稻HSP82;WRKY68:水稻OsWRKY68。下同。An:花药;Bt:孕穗期;Fw:开花期;Husk:颖壳;Low:叶片下部;Mid:叶片中部;MP:成熟穗;PA:穗轴;Rt:幼根;Ti:分蘖期;Up:叶片上部
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Fig. 1. Expression profiling of OsWRKY68 protein in different tissues at different developmental stages WB conditions: Sample loading volume 10 μL. Electrophoresis: 80 V, 20 min, 160 V, 90 min, Gel concentration 10% tricine. Electrophoresis apparatus Mini PROTEAN Tetra cell. Membrane (PVDF) transfer: 100 V, 60 min, Primary Antibody: anti-OsWRKY68 polyclonal antibody (BPI, AbP80049-A-S), anti-HSP82 monoclonal antibody (BPI, AbM51099-31-PU), Secondary antibody: goat anti-rabbit secondary antibody (BPI, AbP-71001-D-HRP), goat anti-mouse secondary antibody (BPI, AbP-71003-D-HRP). HSP: Rice OsHSP82; WRKY68: Rice OsWRKY68. The same as below. An: Anther; Bt: Booting; Fw: Flowering; Low: Lower part of leave; Mid: Middle part of leave; MP: Mature panicle; PA: Panicle axis; Rt: Root; Ti: Tillering; Up: Upper part of leave
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