广西玉米穗腐病致病镰孢种群构成与毒素化学型分析

杜青,唐照磊,李石初,上官玲玲,李华娇,段灿星

Composition of Fusarium Species Causing Maize Ear Rot and Analysis of Toxigenic Chemotype in Guangxi

DU Qing,TANG ZhaoLei,LI ShiChu,SHANGGUAN LingLing,LI HuaJiao,DUAN CanXing

表1 镰孢菌种特异性鉴定引物

Table 1 Specific primers for Fusarium spp. identification

检测真菌 Detected fungus 引物 Primer 引物序列 Primer sequence (5′-3′) 扩增片段 Target fragment (bp) 退火温度 Tm (℃) 镰孢菌 ItsF AACTCCCAAACCCCTGTGAACATA 431 58 Fusarium spp. ItsR TTTAACGGCGTGGCCGC 禾谷镰孢复合种 Fg16NF ACAGATGACAAGATTCAGGCACA 280 57 F. graminearum Fg16NR TTCTTTGACATCTGTTCAACCCA species complex 拟轮枝镰孢 VER1 CTTCCTGCGATGTTTCTCC 578 56 F. verticillioides VER2 AATTGGCCATTGGTATTATATATCTA 层出镰孢 PRO1 CTTTCCGCCAAGTTTCTTC 585 56 F. proliferatum PRO2 TGTCAGTAACTCGACGTTGTTG