家蚕微孢子虫极管蛋白2（NbPTP2）的表达、纯化和定位特征
易敏1,2,吕青1,刘柯柯1,王礼君1,吴玉娇1,周泽扬1,龙梦娴1,2(
)
)
Expression, Purification and Localization Analysis of Polar Tube Protein 2 (NbPTP2) from Nosema bombycis
YI Min1,2,LÜ Qing1,LIU KeKe1,WANG LiJun1,WU YuJiao1,ZHOU ZeYang1,LONG MengXian1,2()
)
图5. 间接免疫荧光分析NbPTP2在家蚕微孢子虫中的定位
A—D：阴性对照弹出的极管与阴性血清没有荧光反应,且发芽的孢子中孢原质被输送到孢子外,已检测不到细胞核The negative control showed no fluorescence reaction between the discharged polar tube and the negative serum, and the sporogen was transported outside, the fluorescence could not be detected;A、E：DIC观察DIC observation;B、F：DAPI染色观察DAPI staining observation;C、G：Alexa 594标记荧光观察Alexa 594 fluorescent observation;D、H：叠加图Merged figures。白色箭头所示为极管,蓝色荧光为经DAPI染色的细胞核The polar tube was indicated by white arrow, and blue fluorescence was nuclear stained by DAPI
Fig. 5. Indirect immunofluorescence assay analysis of NbPTP2 localization in N. bombycis
