家蚕微孢子虫极管蛋白2（NbPTP2）的表达、纯化和定位特征
易敏1,2,吕青1,刘柯柯1,王礼君1,吴玉娇1,周泽扬1,龙梦娴1,2(
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Expression, Purification and Localization Analysis of Polar Tube Protein 2 (NbPTP2) from Nosema bombycis
YI Min1,2,LÜ Qing1,LIU KeKe1,WANG LiJun1,WU YuJiao1,ZHOU ZeYang1,LONG MengXian1,2()
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图3. SDS-PAGE检测pET32a(+)-NbPTP2重组蛋白的诱导表达及纯化
M：蛋白Marker Protein Marker;1：未诱导的pET32a(+)-PTP2重组菌蛋白Uninduced recombinant bacterial protein of pET32a(+)-PTP2;2：诱导的pET32a(+)-PTP2重组菌蛋白Induced recombinant bacterial protein of pET32a(+)-PTP2;3：超声破碎后的pET32a(+)-PTP2重组菌上清The supernatant of pET32a(+)-PTP2 recombinant bacteria after ultrasonic breaking;4：超声破碎后的pET32a(+)-PTP2重组菌沉淀The precipitation of pET32a(+)-PTP2 recombinant bacteria after ultrasonic breaking;5：纯化后的融合蛋白Purified fusion protein
Fig. 3. SDS-PAGE analysis of expression and purification of pET32a(+)-NbPTP2 recombinant protein
