家蚕微孢子虫极管蛋白2（NbPTP2）的表达、纯化和定位特征
易敏1,2,吕青1,刘柯柯1,王礼君1,吴玉娇1,周泽扬1,龙梦娴1,2(
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Expression, Purification and Localization Analysis of Polar Tube Protein 2 (NbPTP2) from Nosema bombycis
YI Min1,2,LÜ Qing1,LIU KeKe1,WANG LiJun1,WU YuJiao1,ZHOU ZeYang1,LONG MengXian1,2()
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图2. pET32a(+)-NbPTP2重组质粒的PCR及双酶切验证
M：DL2000 Plus Marker;1：pET32a(+)-NbPTP2重组质粒的PCR产物 PCR product of pET32a(+)-NbPTP2 recombinant plasmid;2：pET32a(+)- NbPTP2重组质粒的双酶切产物Double enzyme digestion product of pET32a(+)- NbPTP2 recombinant plasmid
Fig. 2. PCR and enzyme digestion analysis of pET32a(+)- NbPTP2 recombinant plasmid
