家蚕微孢子虫极管蛋白2（NbPTP2）的表达、纯化和定位特征

易敏,吕青,刘柯柯,王礼君,吴玉娇,周泽扬,龙梦娴

Expression, Purification and Localization Analysis of Polar Tube Protein 2 (NbPTP2) from Nosema bombycis

YI Min,LÜ Qing,LIU KeKe,WANG LiJun,WU YuJiao,ZHOU ZeYang,LONG MengXian

表1 LC-MS/MS质谱分析融合蛋白

Table 1 LC-MS/MS analysis of recombinant protein

蛋白 Protein 等电点/相对分子质量 PI/MW (kD) 唯一肽段数 UniquePepCount 肽段覆盖率 CoverPercent 肽信息Peptide information 起始位点 Start site 结束位点 End site 序列 Sequence Polar tube protein 2 （EOB15197.1） 9.39/30.9 23 75.09% 1 8 MFLSLNRK 8 21 KLFTVASLLTVIRT 20 38 RTMASVAPPQGGIMVPNRA 37 51 RAQQQMLPAVVDPRK 51 63 KAVCNIQYINGKK 63 78 KIIPANNNNPAECQRD 77 83 RDLIERA 86 97 RQAQAIETAARA 96 106 RAVQTIEHIKQ 96 110 RAVQTIEHIKQEPKC 117 124 RETQCIKT 125 132 KLLNELKN 125 145 KLLNELKNEPEYTVTGEENKV 131 145 KNEPEYTVTGEENKV 144 150 KVNVFKK 144 151 KVNVFKKG 152 167 KKIMLAANIQHHFIKV 153 167 KIMLAANIQHHFIKV 166 180 KVEKPPSEQFQIVKK 180 198 KAKEAYVFNAIGEVLSTKQ 201 213 KRPAPQCICPGKS 212 229 KSTPQTAEGTPLINECKQ 228 234 KQAECKN