SMAD1基因的沉默和过表达及对秦川牛原代成肌细胞生肌的影响
宁越1,米雪1,陈星伊1,邵建航1,昝林森1,2(
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Silencing and Overexpressing SMAD Family Member 1 (SMAD1) Gene and Its Effect on Myogenesis in Primary Myoblast of Qinchuan Cattle (Bos taurus)
NING Yue1,MI Xue1,CHEN XingYi1,SHAO JianHang1,ZAN LinSen1,2()
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图6. 沉默、过表达SMAD1基因调控牛原代成肌细胞分化
A：转染siSMAD1并诱导分化6天时牛原代成肌细胞分化融合情况;B：侵染腺病毒AD-bSMAD1并成肌诱导分化6天时秦川牛原代成肌细胞分化融合情况;C：沉默SMAD1后肌分化标志基因的mRNA水平检测;D：过表达SMAD1后肌分化标志基因的mRNA水平检测。原始放大倍数：40× (OLYMPUS IX71, 比例尺：200 μm)
Fig. 6. Regulation of bovine primary myoblasts differentiation by silencing and overexpressing SMAD1 gene
A: Cell micrographs of bovine primary myoblasts after 6 days induction transfected with siSMAD1; B: Cell micrographs of bovine primary myoblasts after 6 days induction infected with AD-bSMAD1; C: Relative mRNA expression of MyoD, Myf5 and MyoG after silencing SMAD1 gene; D: Relative mRNA expression of MyoD, Myf5 and MyoG after overexpression of SMAD1 gene. Original magnification: 40× (OLYMPUS IX71, scale bars: 200 μm)
