SMAD1基因的沉默和过表达及对秦川牛原代成肌细胞生肌的影响
宁越1,米雪1,陈星伊1,邵建航1,昝林森1,2(
)
)
Silencing and Overexpressing SMAD Family Member 1 (SMAD1) Gene and Its Effect on Myogenesis in Primary Myoblast of Qinchuan Cattle (Bos taurus)
NING Yue1,MI Xue1,CHEN XingYi1,SHAO JianHang1,ZAN LinSen1,2()
)
图4. 重组腺病毒AD-bSMAD1的包装与扩繁
A：转染目的质粒到293细胞24h,GFP表达情况;B：转染9天后,病变细胞开始出毒,绿色荧光呈现葡萄串样的聚集现象;C：AD-bSMAD1转染14d以及AD-NC转染12d时,大部分细胞收缩变圆,开始从培养瓶壁脱落,将其收集为病毒母液P1;D：高滴度病毒（P3）侵染293细胞48h,GFP表达情况。原始放大倍数：40× (OLYMPUS IX71, 比例尺：200 μm)
Fig. 4. Adenovirus packaging and amplification
A: GFP Expression of 293 cells infected after 24 h; B: Green fluorescence showed a grapes-like gathering and adenovirus started to appear after 9 d; C: After 14d-infection of AD-bSMAD1 and 12d-infection of AD-NC, most cells became shrinkage, roundout and shedding, gathered as the first-generation adenovirus; D: GFP expression of 293 cells after 48h-infection of high titers adenovirus (P3). Original magnification: 40× (OLYMPUS IX71, scale bars: 200 μm)
