SMAD1基因的沉默和过表达及对秦川牛原代成肌细胞生肌的影响
宁越1,米雪1,陈星伊1,邵建航1,昝林森1,2(
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Silencing and Overexpressing SMAD Family Member 1 (SMAD1) Gene and Its Effect on Myogenesis in Primary Myoblast of Qinchuan Cattle (Bos taurus)
NING Yue1,MI Xue1,CHEN XingYi1,SHAO JianHang1,ZAN LinSen1,2()
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图3. 秦川牛SMAD1基因腺病毒载体pDC316-mCMV-EGFP-bSMAD1的构建
A：秦川牛SMAD1基因CDS区序列PCR产物;B：pDC316-mCMV-EGFP-bSMAD1质粒酶切鉴定结果。M1：DL2000 DNA marker;M2：Trans2K plus marker;泳道1,2为SMAD1基因在两种成肌细胞cDNA中的PCR产物;泳道3为pDC316-mCMV-EGFP-bSMAD1的NheI和NotI双酶切鉴定结果
Fig. 3. Construction of the adenovirus vector pDC316-mCMV-EGFP-bSMAD1
A: PCR products of SMAD1 gene CDS region in Qinchuan cattle; B: Identification of recombinant plasmid pDC316-mCMV-EGFP-bSMAD1 by double digestion of NheI and NotI. M1: DL2000 DNA marker; M2: Trans2K plus marker; lane1 and 2: PCR products of SMAD1 gene in two kinds of myoblasts; lane 3: Identification of pDC316-mCMV-EGFP-bSMAD1 by double digestion of NheI and NotI
