SMAD1基因的沉默和过表达及对秦川牛原代成肌细胞生肌的影响

宁越,米雪,陈星伊,邵建航,昝林森

Silencing and Overexpressing SMAD Family Member 1 (SMAD1) Gene and Its Effect on Myogenesis in Primary Myoblast of Qinchuan Cattle (Bos taurus)

NING Yue,MI Xue,CHEN XingYi,SHAO JianHang,ZAN LinSen

表4 病毒浓度梯度

Table 4 Concentration gradient of adenovirus

稀释倍数 Diluted multiples 上一梯度病毒液 Virus of upper gradient DMEM完培 DMEM complete medium 上样量 Volume of the sample 滴定量 Volume of the virus 103 10 90 10 1μL 104 10 90 10 0.1μL 105 10 90 10 0.01μL 106 10 90 10 0.001μL 107 10 90 10 0.0001μL … … … … …