SMAD1基因的沉默和过表达及对秦川牛原代成肌细胞生肌的影响
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宁越,米雪,陈星伊,邵建航,昝林森
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Silencing and Overexpressing SMAD Family Member 1 (SMAD1) Gene and Its Effect on Myogenesis in Primary Myoblast of Qinchuan Cattle (Bos taurus)
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NING Yue,MI Xue,CHEN XingYi,SHAO JianHang,ZAN LinSen
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表3 PCR引物序列
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Table 3 PCR Primers
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| 基因名称 Gene name | 登录号 Accession No. | 引物序列 （5'→3'） Primer sequences (5'→3') | 产物 Size（bp） | | CDS-SMAD1 | NM_001077107.2 | F:CTAGCTAGCCACCATGAATGTGACAAGTTTATTTTCCTTCAC | 1452 | | R:ATTTGCGGCCGCCTACAGATCCTCTTCTGAGATGAGTTTTTGTTCC | | RT-SMAD1 | NM_001077107.2 | F:TTCGCATGAGCTTTGTGAAG | 98 | | R:GTGAGCCCATCTGGGTAAGA | | RT-MyoD | NM_001040478 | F:TCCGCGACGTAGACTTGA | 84 | | R:CGAAACACGGGTCATCATAGAA | | RT-MyoG | NM_001111325 | F:GGCGTGTAAGGTGTGTAAG | 85 | | R:CTTCTTGAGTCTGCGCTTCT | | RT-Myf5 | NM_174116.1 | F:CCTCTAGTTCCAGGCTCATCTA | 90 | | R:ACCTCCTTCCTCCTGTGTAATA | | RT-GAPDH | NM_001034034 | F: CCAACGTGTCTGTTGTGGAT | 80 | | R: CTGCTTCACCACCTTCTTGA |
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