SMAD1基因的沉默和过表达及对秦川牛原代成肌细胞生肌的影响

宁越,米雪,陈星伊,邵建航,昝林森

Silencing and Overexpressing SMAD Family Member 1 (SMAD1) Gene and Its Effect on Myogenesis in Primary Myoblast of Qinchuan Cattle (Bos taurus)

NING Yue,MI Xue,CHEN XingYi,SHAO JianHang,ZAN LinSen

表2 siRNA序列

Table 2 Sequences of siRNA

序列名称 Sequence name	序列（5'→3'） Sequences (5'→3')
siSMAD1	sense：5'-CCAUCUCUGCCUUCAGAAATT-3'
siSMAD1	antisense：5'-UUUCUGAAGGCAGAGAUGGTT-3'
siRNA-NC	sense：5'-UUCUCCGAACGUGUCACGUTT-3'
siRNA-NC	antisense：5'-ACGUGACACGUUCGGAGAATT-3'