茶树咖啡碱合成酶基因稀有等位变异TCS1g的筛选、克隆及功能
刘玉飞1,2,金基强1,姚明哲1(
),陈亮1()
),陈亮1()
Screening, Cloning and Functional Research of the Rare Allelic Variation of Caffeine Synthase Gene (TCS1g) in Tea Plants
LIU YuFei1,2,JIN JiQiang1,YAO MingZhe1(),CHEN Liang1()
),CHEN Liang1()
图6. 实时定量荧光PCR分析TCS1g表达水平
TCS1-CS,TCS1-TS和TCS1-Total分别表示表达蛋白同时具有可可碱和咖啡碱合成酶活性的等位变异、表达蛋白仅具有可可碱合成酶活性的等位变异和整体TCS1的表达水平
Fig. 6. Real-time PCR was used to analyze TCS1g specific expression
TCS1-CS, TCS1-TS, and TCS1-Total are the expression levels of allelic variation encoding proteins with both theobromine synthase (TS) and caffeine synthase (CS) activities, only with TS activities, and total TCS1, respectively
