茶树咖啡碱合成酶基因稀有等位变异TCS1g的筛选、克隆及功能
刘玉飞1,2,金基强1,姚明哲1(),陈亮1()
Screening, Cloning and Functional Research of the Rare Allelic Variation of Caffeine Synthase Gene (TCS1g) in Tea Plants
LIU YuFei1,2,JIN JiQiang1,YAO MingZhe1(),CHEN Liang1()

图4. TCS1g与TCS1其他6个等位变异的氨基酸序列比对
SAM的结合域A、B和C以及一个保守域YFFF用红框标出[15,28-29]。与底物特异性结合的关键位点用篮框标出[15,17,30]。TCS1b、TCS1c、TCS1g相比于TCS1a、TCS1d、TCS1e、TCS1f的变异位点用棕黄色三角标出

Fig. 4. Comparison of amino acid sequences of TCS1g and other six allelic variants of TCS1
The proposed SAM-binding motifs (A, B’, and C) and conserved region that is nominated as ‘‘YFFF-region’’ are shown by red open boxes [15,28-29]. The amino acid residues indicated by a blue open box play a critical role in substrate recognition [15,17,30]. The TCS1b, TCS1c and TCS1 g variant sites compared to TCS1a, TCS1d, TCS1e and TCS1f are indicated by a brownish yellow triangle