茶树咖啡碱合成酶基因稀有等位变异TCS1g的筛选、克隆及功能
刘玉飞1,2,金基强1,姚明哲1(
),陈亮1()
),陈亮1()
Screening, Cloning and Functional Research of the Rare Allelic Variation of Caffeine Synthase Gene (TCS1g) in Tea Plants
LIU YuFei1,2,JIN JiQiang1,YAO MingZhe1(),CHEN Liang1()
),CHEN Liang1()
图1. 咖啡碱生物合成的核心途径
（1）7-甲基黄嘌呤核苷合成酶（黄嘌呤核苷N-甲基转移酶）;（2）N-甲基核苷酶;（3）可可碱合成酶（单甲基黄嘌呤N-甲基转移酶）：TCS1b（ICS1）、TCS1c（PCS1）;（4）咖啡碱合成酶（二甲基黄嘌呤N-甲基转移酶）;（3-4）双功能咖啡碱合成酶：TCS1a、TCS1d、TCS1e、TCS1f。SAM：S-腺苷-L-甲硫氨酸;SAH：S-腺苷-L-高半胱氨酸;Ribose：核糖。图片参考文献[
Fig. 1. The major biosynthetic pathway of caffeine synthesis
(1) 7-methylxanthosine synthase (xanthosine N-methyltransferase); (2) N-methylnucleosidase; (3) theobromine synthase (monomethylxanthine N-methyltransferase; TS): ICS1 (TCS1b), PCS1 (TCS1c); (4) caffeine synthase (dimethylxanthine N-methyltransferase; CS); (3-4) dual-functional caffeine synthase: TCS1a, TCS1d, TCS1e, TCS1f. SAM, S-adenosyl-Lmethionine; SAH, S-adenosyl-L-homocysteine. The picture refers to references [10-12]
