茶树咖啡碱合成酶基因稀有等位变异TCS1g的筛选、克隆及功能

刘玉飞,金基强,姚明哲,陈亮

Screening, Cloning and Functional Research of the Rare Allelic Variation of Caffeine Synthase Gene (TCS1g) in Tea Plants

LIU YuFei,JIN JiQiang,YAO MingZhe,CHEN Liang

表3 TCS1g重组蛋白活性分析

Table 3 Comparison of the activity and substrate selectivity of TCS1g recombinant enzymes

重组蛋白 Recombinant enzyme 底物/甲基化位置 Substrate/Methylation position Tb/7-mX (%) 7-mX/3-N (pkat/mg) Tb/1-N (pkat/mg) TCS1a 111.9±5.0 27.1±0.1 24.2±1.1 TCS1c 17.9±0.9 ND 0 TCS1g 44.3±0.3 ND 0