茶树咖啡碱合成酶基因稀有等位变异TCS1g的筛选、克隆及功能

刘玉飞,金基强,姚明哲,陈亮

Screening, Cloning and Functional Research of the Rare Allelic Variation of Caffeine Synthase Gene (TCS1g) in Tea Plants

LIU YuFei,JIN JiQiang,YAO MingZhe,CHEN Liang

表1 试验引物及其序列

Table 1 Primer sequences used in study

引物名称 Primer name 引物序列Primer sequence (5′-3′) TCS1P InDel F TATGTCATGTTTCTATTATTT TCS1P InDel R TACTTTCTCCTTCTCCTCTGT TCS1cDNAF CACTGCTGTGGCAGCTGGC TCS1cDNAR CAACTTCTCATTTCTCCCAAC TCS1gF CGGGATCCATGGGGAAGGTGAACGAAG TCS1gR CGGAATTCCTATCCAACAATCTTGGAA 18S-F TCTCAACCATAAACGATGCCGACCAG 18S-R TTTCAGCCTTGCGACCATACTCCC TCS1-CS-F TTACTTTTCTGACGAGGCA TCS1-CS-R TGCGTTAAGAGCTTGAAGG TCS1-TS-F ATTACTTTTCTCCGACGAGGCG TCS1-TS-R TGCAGTAAGAGCTTGAAGAA TCS1-Total-F AGCTGGCCTCTTTGCYATAA TCS1-Total-R ACTAYTTTCTCCTTCTCCTC