甜菜夜蛾卵黄原蛋白受体基因的RNA干扰

赵静,陶蓉,郝德君,肖留斌,谭永安

RNA Interference of Vitellogenin Receptor Gene in Beet Armyworm (Spodoptera exigua)

ZHAO Jing,TAO Rong,HAO DeJun,XIAO LiuBin,TAN YongAn

表1 本研究所用引物

Table 1 Primers used in this study

引物名称Primer name 引物序列 Primer sequence (5′ to 3′) 产物长度Product length (bp) dsRNA VgR-F CACAATCAAGACCGATACCA 327 VgR-R GTCCAGTCACTCCAGAACACT VgR-TF TAATACGACTCACTATAGGG CACAATCAAGACCGATACCA VgR-TR TAATACGACTCACTATAGGG GTCCAGTCACTCCAGAACACT GFP-F CACAAGTTCAGCGTGTCCG 417 GFP-R CACCTTGATGCCGTTC GFP-TF TAATACGACTCACTATAGGG CACAAGTTCAGCGTGTCCG GFP-TR TAATACGACTCACTATAGGG CACCTTGATGCCGTTC RT-qPCR VgR-QF GAAGGGAGGGAAGTGTCCTGAG 104 VgR-QR TGATGGTGAAAGAAACGCTGTG β-actin-F CCAGCCTTCCTTCTTGGGTAT 93 β-actin -R AGGTCCTTACGGATGTCAACG